大鼠主动脉球囊内皮剥脱对血小板活化功能的影响

目的：观察大鼠主动脉球囊内皮剥脱对血小板活化的影响。方法：在大鼠主动脉球囊内皮剥脱模型上，分别于术后7、14和21天观察球囊内皮剥脱对血小板活化的影响。结果：大鼠球囊内皮剥脱术后，于7、14、21天时，血小板聚集明显增强，蛋白激酶（PKC）活性明显增高，环磷酸腺苷（cAMP）活性明显降低，均以7天组最为明显。结论：临床PTCA后较长时间应用抗栓剂是必要的。     

The cytotoxicity of eosinophil cationic protein/ribonuclease 3 on eukaryotic cell lines takes place through its aggregation on the cell membrane

Human eosinophil cationic protein (ECP)/ ribonuclease 3 (RNase 3) is a protein secreted from the secondary granules of activated eosinophils. Specific properties of ECP contribute to its cytotoxic activities associated with defense mechanisms. In this work the ECP cytotoxic activity on eukaryotic cell lines is analyzed. The ECP effects begin with its binding and aggregation to the cell surface, altering the cell membrane permeability and modifying the cell ionic equilibrium. No internalization of the protein is observed. These signals induce cell-specific morphological and biochemical changes such as chromatin condensation, reversion of membrane asymmetry, reactive oxygen species production and activation of caspase-3-like activity and, eventually, cell death. However, the ribonuclease activity component of ECP is not involved in this process as no RNA degradation is observed. In summary, the cytotoxic effect of ECP is attained through a mechanism different from that of other cytotoxic RNases and may be related with the ECP accumulation associated with the inflammatory processes, in which eosinophils are present. Received 26 October 2007; accepted 23 November 2007     

ERK activation by Ca2+ ionophores depends on Ca2+ entry in lymphocytes but not in platelets, and does not conduct membrane scrambling

Rapid Ca²⁺-dependent phospholipid (PL) reorganization (scrambling) at the plasma membrane is a mechanism common to hematopoietic cells exposing procoagulant phosphatidylserine (PS). The aim of this research was to determine whether activation of the extracellular signal-regulated kinase (ERK) pathway was required for PL scrambling, based on a single report analyzing both responses induced by Ca²⁺ ionophores in megakaryoblastic HEL cells. Ca²⁺ ionophore-stimulated ERK phosphorylation was induced in platelets without external Ca²⁺, whereas exogenous Ca²⁺ entry was crucial for ERK activation in Jurkat T cells. In both cells, membrane scrambling only occurred following Ca²⁺ entry and was not blocked by inhibiting ERK phosphorylation. Furthermore, ERK proteins are strongly phosphorylated in transformed B lymphoblastic cell lines, which do not expose PS in their resting state. Overall, the data demonstrated that ERK activation and membrane scrambling are independent mechanisms. A. Arachiche, I. Badirou: These authors contributed equally to this work. Received 18 June 2008; received after revision 24 September 2008; accepted 1 October 2008     

一种综合评价指标属性值规一量化技术新途径

对现有的评价指标属性值规一量化技术进行了论述 ,运用模式识别、人工神经网络聚类理论结合模糊集值统计理论 ,提出了评价指标属性值规一量化技术新途径 ,奠定了评价指标属性值规一量化技术智能化的基础 .     

表面活性剂存在时甲苯胺蓝聚合状态光谱分析

对生化试剂－甲苯胺蓝水溶液的吸收光谱进行了研究，测定出以波长为６６５ｎｍ和６１０ｎｍ处有两个最大吸收峰，证明了ＴＢ在水溶液中存在着单体与双分子聚集体的解离平衡。其二聚体的含量与溶液的浓度成正比。     

一种基于时序感知的社会网络节点集情境聚合方法

情境聚合是社会感知计算的主要研究工作之一,目前主要采用基于特征提取的方法,对社会的交流方式进行量化分析.但是大多数的研究中忽略了情境信息的时序性,针对社会感知计算中时序情境信息的聚合问题,首先对时序情境和计算情境的聚合过程进行探讨,提出基于时序扩展的计算情境元素,用于情境对象的聚合,并给出一种时序感知的社会网络节点集情境聚合方法.实验表明,该方法能够根据时间情境和计算情境元素的特点,把聚合对象划分为相对情境聚合对象和绝对情境对象,有效地对社会环境中的时序信息进行情境聚合.     

确定相对决策权重的链比较法

基于层次分析法(analytichierarchyprocess,AHP)的基本原理,在假定决策者两两比较判断矩阵已知的情况下,提出了一种根据两两比较判断矩阵确定决策者对决策对象的综合决策权重向量的新方法———链比较法,并用一个数值例示进行了说明。最后,讨论了链比较法的优点与可能的不足以及可以进一步改进的地方,与其他方法进行了比较,说明了方法的合理性,并就AHP未来可能的研究问题进行了展望。     

金融波动的平方根随机自回归波动模型

讨论了SR-SARV模型的时间聚合性和同期聚合性,比较了波动模型之间的关系,指出了SR-SARV模型研究的意义,并给出其参数估计方法,对上证指数进行了实证研究。     

赤芍总苷对大鼠血小板功能的影响

为了研究赤芍总苷对血小板聚集功能的影响,采用比浊法观察赤芍总苷对大鼠血小板聚集功能的作用,用放射免疫法观察对大鼠TXB2/6-keto-PGF1α、ET水平的作用,并用硝酸还原酶法测定大鼠血清中NO的浓度。结果发现,赤芍总苷能降低ADP诱导的血小板最大聚集强度,降低大鼠血浆中TXB2的浓度,同时升高血浆中6-keto-PGF1α的水平,调节大鼠血清NO/ET的平衡。实验证明,赤芍总苷有明显降低大鼠血小板聚集的作用。     

小儿原发性血小板减少性紫癜治疗进展

近年来，对于小儿原发性血小板减少性紫癜（ITP）的治疗，不断推出各种新疗法。笔者就各种疗法治疗ITP的进展进行了综述。